Cell-free Embryonic Stem Cell Extract-Mediated Derivation of Multi-potent Stem Cells from NIH3T3 Fibroblasts for Functional and Anatomical Ischemic Tissue Repair
Identifieur interne : 006B91 ( Main/Exploration ); précédent : 006B90; suivant : 006B92Cell-free Embryonic Stem Cell Extract-Mediated Derivation of Multi-potent Stem Cells from NIH3T3 Fibroblasts for Functional and Anatomical Ischemic Tissue Repair
Auteurs : Johnson Rajasingh [États-Unis] ; Erin Lambers [États-Unis] ; Hiromichi Hamada [États-Unis] ; Evelyn Bord [États-Unis] ; Tina Thorne [États-Unis] ; Ilona Goukassian [États-Unis] ; Prasanna Krishnamurthy [États-Unis] ; Kenneth M. Rosen [États-Unis] ; Deepali Ahluwalia [États-Unis] ; Yan Zhu [États-Unis] ; Gangjian Qin [États-Unis] ; Douglas W. Losordo [États-Unis] ; Raj Kishore [États-Unis]Source :
- Circulation research [ 0009-7330 ] ; 2008.
Abstract
The oocyte-independent source for the generation of pluripotent stem cells is one of the ultimate goals in regenerative medicine. We report that upon exposure to mouse ES cell (ESC) extracts, reversibly permeabilized NIH3T3 cells undergo de-differentiation followed by stimulus-induced re-differentiation into multiple lineage cell types. Genome-wide expression profiling revealed significant differences between NIH3T3 control and ESC extract treated NIH3T3 cells including the re-activation of ESC specific transcripts. Epigenetically, ESC extracts induced CpG de-methylation of Oct4 promoter, hyper-acetylation of histones 3 and 4 and decreased lysine 9 (K-9) dimethylation of histone 3. In mouse models of surgically-induced hind limb ischemia (HLI) or acute myocardial infarction (AMI) transplantation of reprogrammed NIH3T3 cells significantly improved post-injury physiological functions and showed anatomical evidence of engraftment and trans-differentiation into skeletal muscle, endothelial cell and cardiomyocytes. These data provide evidence for the generation of functional multi-potent stem like cells from terminally differentiated somatic cells without the introduction of retroviral mediated trans-genes or ESC fusion.
Url:
DOI: 10.1161/CIRCRESAHA.108.176115
PubMed: 18483406
PubMed Central: 2435186
Affiliations:
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Tufts University School of Medicine, Boston</wicri:cityArea></affiliation></author><author><name sortKey="Ahluwalia, Deepali" sort="Ahluwalia, Deepali" uniqKey="Ahluwalia D" first="Deepali" last="Ahluwalia">Deepali Ahluwalia</name><affiliation wicri:level="2"><nlm:aff id="A1">Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue, Chicago IL 60611</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue</wicri:cityArea></affiliation></author><author><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name><affiliation wicri:level="2"><nlm:aff id="A2">Division of Neurology Research, Caritas St. Elizabeth's Medical Center. Tufts University School of Medicine, Boston, MA 02135</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Massachusetts</region></placeName><wicri:cityArea>Division of Neurology Research, Caritas St. Elizabeth's Medical Center. Tufts University School of Medicine, Boston</wicri:cityArea></affiliation></author><author><name sortKey="Qin, Gangjian" sort="Qin, Gangjian" uniqKey="Qin G" first="Gangjian" last="Qin">Gangjian Qin</name><affiliation wicri:level="2"><nlm:aff id="A1">Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue, Chicago IL 60611</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue</wicri:cityArea></affiliation></author><author><name sortKey="Losordo, Douglas W" sort="Losordo, Douglas W" uniqKey="Losordo D" first="Douglas W." last="Losordo">Douglas W. Losordo</name><affiliation wicri:level="2"><nlm:aff id="A1">Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue, Chicago IL 60611</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue</wicri:cityArea></affiliation></author><author><name sortKey="Kishore, Raj" sort="Kishore, Raj" uniqKey="Kishore R" first="Raj" last="Kishore">Raj Kishore</name><affiliation wicri:level="2"><nlm:aff id="A1">Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue, Chicago IL 60611</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue</wicri:cityArea></affiliation></author></analytic><series><title level="j">Circulation research</title><idno type="ISSN">0009-7330</idno><idno type="eISSN">1524-4571</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">The oocyte-independent source for the generation of pluripotent stem cells is one of the ultimate goals in regenerative medicine. We report that upon exposure to mouse ES cell (ESC) extracts, reversibly permeabilized NIH3T3 cells undergo de-differentiation followed by stimulus-induced re-differentiation into multiple lineage cell types. Genome-wide expression profiling revealed significant differences between NIH3T3 control and ESC extract treated NIH3T3 cells including the re-activation of ESC specific transcripts. Epigenetically, ESC extracts induced CpG de-methylation of Oct4 promoter, hyper-acetylation of histones 3 and 4 and decreased lysine 9 (K-9) dimethylation of histone 3. In mouse models of surgically-induced hind limb ischemia (HLI) or acute myocardial infarction (AMI) transplantation of reprogrammed NIH3T3 cells significantly improved post-injury physiological functions and showed anatomical evidence of engraftment and trans-differentiation into skeletal muscle, endothelial cell and cardiomyocytes. These data provide evidence for the generation of functional multi-potent stem like cells from terminally differentiated somatic cells without the introduction of retroviral mediated trans-genes or ESC fusion.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Illinois</li><li>Massachusetts</li></region></list><tree><country name="États-Unis"><region name="Illinois"><name sortKey="Rajasingh, Johnson" sort="Rajasingh, Johnson" uniqKey="Rajasingh J" first="Johnson" last="Rajasingh">Johnson Rajasingh</name></region><name sortKey="Ahluwalia, Deepali" sort="Ahluwalia, Deepali" uniqKey="Ahluwalia D" first="Deepali" last="Ahluwalia">Deepali Ahluwalia</name><name sortKey="Bord, Evelyn" sort="Bord, Evelyn" uniqKey="Bord E" first="Evelyn" last="Bord">Evelyn Bord</name><name sortKey="Goukassian, Ilona" sort="Goukassian, Ilona" uniqKey="Goukassian I" first="Ilona" last="Goukassian">Ilona Goukassian</name><name sortKey="Hamada, Hiromichi" sort="Hamada, Hiromichi" uniqKey="Hamada H" first="Hiromichi" last="Hamada">Hiromichi Hamada</name><name sortKey="Kishore, Raj" sort="Kishore, Raj" uniqKey="Kishore R" first="Raj" last="Kishore">Raj Kishore</name><name sortKey="Krishnamurthy, Prasanna" sort="Krishnamurthy, Prasanna" uniqKey="Krishnamurthy P" first="Prasanna" last="Krishnamurthy">Prasanna Krishnamurthy</name><name sortKey="Lambers, Erin" sort="Lambers, Erin" uniqKey="Lambers E" first="Erin" last="Lambers">Erin Lambers</name><name sortKey="Losordo, Douglas W" sort="Losordo, Douglas W" uniqKey="Losordo D" first="Douglas W." last="Losordo">Douglas W. Losordo</name><name sortKey="Qin, Gangjian" sort="Qin, Gangjian" uniqKey="Qin G" first="Gangjian" last="Qin">Gangjian Qin</name><name sortKey="Rosen, Kenneth M" sort="Rosen, Kenneth M" uniqKey="Rosen K" first="Kenneth M." last="Rosen">Kenneth M. Rosen</name><name sortKey="Thorne, Tina" sort="Thorne, Tina" uniqKey="Thorne T" first="Tina" last="Thorne">Tina Thorne</name><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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